Spectral fluorometric characterization of phytoplankton types in the tropical coastal waters of Singapore

摘要: Abstract Developing rapid in situ optical detection techniques for discriminating the potential harmful algal blooms is an ongoing goal scientists around world. The utility of a profiling 9-wavelength Multi-Exciter fluorometer concert with other bio-optical measurements was examined using isolate cultures laboratory and field survey East Johor Strait, Singapore. Background confirmed difficulty discerning between diatoms dinoflagellates, although raphidophytes dinoflagellate Karlodinium sp. did show unique signatures. Significant relationships were found 470 nm signal traditional chlorophyll pigment both ( p specific-absorbance -fluorescence showed identifying phytoplankton groups based on slope relationship them. 6-station Strait displayed relatively shallow depths (5.06 ± 0.81 m), warm temperatures (29.88 ± 0.26 °C), low salinity (25.81 ± 1.48) high biomass (17.6 ± 11.7 μg Chl-  L − 1 ). Absorbance fluorometric from not reveal similar signatures studied isolates, 435 nm:570 nm 470 nm:525 nm ratios distinct profile responses. However, these wavelength-to-wavelength ratio identified, further investigation required. results suggest that fluorometry coupled absorption properties could assist defining bloom parameters enhance our ability determining detecting pre-bloom conditions.