Effects of aging and reproduction on protein quality control in soma and gametes of Drosophila melanogaster

摘要: In organisms with a soma-germ demarcation, the germline must be 'preserved' such that harmful damage is not transmitted to offspring. Keeping progeny free of may achieved by gametes enjoying elevated, and/or more functional, homeostatic maintenance systems. This possibility was approached here testing whether soma and maturating oocytes (eggs) dissected from female Drosophila melanogaster in reproductive ages display differential capacities for protein quality control these change during aging mating. Eggs exhibited high capacity prevent aggregation, strong 26S proteasome-dependent degradation reduced levels oxidatively damaged (carbonylated) proteins compared soma. The aggregation affected either or eggs age mating, while proteasome declined but maintained aged females. However, carbonylated increased both eggs, this increase pronounced females allowed mate continuously. Furthermore, mated flies correlated negatively propensity develop into an adult fly. young flies, mating caused decrease carbonylation soma, eggs. These results are line trade-off theories where considered consequence investment reproduction over somatic maintenance.