External quality assessment of enterovirus detection and typing. European Union Concerted Action on Virus Meningitis and Encephalitis.

摘要: Reported are the results of a study an enterovirus proficiency panel for use in isolation and serotyping and/or polymerase chain reaction (PCR) carried out by 12 laboratories nine European countries. Eleven reported virus serotyping. In addition, four PCR detection. Correct were obtained 105 110 samples (95.5%, false-negatives, one false-positive), correct 39 40 (97.5%, false-negative). The highest rate (87.5%) was observed primary tertiary monkey kidney cells; on cell lines, human diploid fibroblasts or heteroploid cells varied between 64% 71.4%. Serotyping less satisfactory. Only 63 106 (59.4%) isolated viruses typed correctly. Major problems seen with containing mixtures enteroviruses 71 echovirus 4, 9%, 50%, 55% results, respectively. These underline need improvement typing, especially view poliomyelitis eradication initiative.