Multiplexed CRISPR technologies for gene editing and transcriptional regulation.
作者： Nicholas S. McCarty , Alicia E. Graham , Lucie Studená , Rodrigo Ledesma-Amaro
DOI: 10.1038/S41467-020-15053-X
关键词: Regulation of gene expression 、 Genome editing 、 Genome engineering 、 CRISPR 、 Computational biology 、 Transcriptional regulation 、 Future studies 、 Guide RNA 、 Biological engineering 、 Computer science
摘要: Multiplexed CRISPR technologies, in which numerous gRNAs or Cas enzymes are expressed at once, have facilitated powerful biological engineering applications, vastly enhancing the scope and efficiencies of genetic editing transcriptional regulation. In this review, we discuss multiplexed technologies describe methods for assembly, expression processing synthetic guide RNA arrays vivo. Applications that benefit from including cellular recorders, circuits, biosensors, combinatorial perturbations, large-scale genome rewiring metabolic pathways, highlighted. We also offer a glimpse emerging challenges emphasize experimental considerations future studies.
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