Combined disc methods for the detection of KPC- and/or VIM-positive Klebsiella pneumoniae: improving reliability for the double carbapenemase producers.
作者： V. Miriagou , E. Tzelepi , S.D. Kotsakis , G.L. Daikos , J. Bou Casals
关键词: Microbiology 、 Aminophenylboronic acid 、 Dipicolinic acid 、 Klebsiella pneumoniae 、 Chemistry 、 Meropenem 、 Phenylboronic acid
摘要: Klebsiella pneumoniae strains co-producing klebsiella carbapenemase (KPC) and verona integron-encoded metallo-beta-lactamase (VIM) are frequently isolated in Greece have also occurred other European countries. Conventional combined disc tests exhibit low sensitivity against these emerging pathogens. We evaluated modifications of the KPC/Metallo-β-Lactamase Confirmation kit (ROSCO) exhibiting high diagnostic value KPC, VIM KPC + producers. The key changes were inclusion additional tablets containing meropenem plus two inhibitors (dipicolinic acid (1000 μg per tablet) for metallo-β-lactamases a boronic derivative KPCs) replacement aminophenylboronic by phenylboronic (400 tablet).
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