Antimicrobial activity and carbohydrate metabolism in the bacterial metagenome of the soil-living invertebrate Folsomia candida

作者: Valeria Agamennone , Ngoc Giang Le , Nico M. van Straalen , Abraham Brouwer , Dick Roelofs

DOI: 10.1038/S41598-019-43828-W

关键词: GeneticsShotgun sequencingGeneMetagenomicsBiologySoil ecologyModel organismSequence assemblyHorizontal gene transferMicrobiome

摘要: The microbiome associated with an animal’s gut and other organs is considered integral part of its ecological functions adaptive capacity. To better understand how microbial communities influence activities capacities the host, we need more information on that are encoded in a microbiome. Until now, about soil invertebrate microbiomes mostly based taxonomic characterization, achieved through culturing amplicon sequencing. Using shotgun sequencing various bioinformatics approaches explored bacterial metagenome Folsomia candida, established model organism ecology fully sequenced, high-quality genome assembly. Our analysis revealed remarkable diversity genes antimicrobial activity carbohydrate metabolism. also contains several homologs to F. candida were previously identified as candidates for horizontal gene transfer (HGT). We suggest carbohydrate- antimicrobial-related by Folsomia’s play role digestion recalcitrant soil-born polysaccharides defense against pathogens, thereby significantly contributing adaptation these animals life soil. Furthermore, from may constitute important source new springtail.

参考文章(116)
Duy Tin Truong, Eric A Franzosa, Timothy L Tickle, Matthias Scholz, George Weingart, Edoardo Pasolli, Adrian Tett, Curtis Huttenhower, Nicola Segata, MetaPhlAn2 for enhanced metagenomic taxonomic profiling Nature Methods. ,vol. 12, pp. 902- 903 ,(2015) , 10.1038/NMETH.3589
Lawrence A Kelley, Stefans Mezulis, Christopher M Yates, Mark N Wass, Michael J E Sternberg, The Phyre2 web portal for protein modeling, prediction and analysis Nature Protocols. ,vol. 10, pp. 845- 858 ,(2015) , 10.1038/NPROT.2015.053
Isabelle J. Schalk, Laurent Guillon, Pyoverdine biosynthesis and secretion in Pseudomonas aeruginosa: implications for metal homeostasis Environmental Microbiology. ,vol. 15, pp. 1661- 1673 ,(2013) , 10.1111/1462-2920.12013
Daniel Antony Pass, Andrew John Morgan, Daniel S. Read, Dawn Field, Andrew J. Weightman, Peter Kille, The effect of anthropogenic arsenic contamination on the earthworm microbiome Environmental Microbiology. ,vol. 17, pp. 1884- 1896 ,(2015) , 10.1111/1462-2920.12712
Tilmann Weber, Kai Blin, Srikanth Duddela, Daniel Krug, Hyun Uk Kim, Robert Bruccoleri, Sang Yup Lee, Michael A. Fischbach, Rolf Müller, Wolfgang Wohlleben, Rainer Breitling, Eriko Takano, Marnix H. Medema, antiSMASH 3.0—a comprehensive resource for the genome mining of biosynthetic gene clusters Nucleic Acids Research. ,vol. 43, pp. 1- 7 ,(2015) , 10.1093/NAR/GKV437
Jinling Huang, Horizontal gene transfer in eukaryotes: The weak‐link model BioEssays. ,vol. 35, pp. 868- 875 ,(2013) , 10.1002/BIES.201300007
Falk Warnecke, Peter Luginbühl, Natalia Ivanova, Majid Ghassemian, Toby H. Richardson, Justin T. Stege, Michelle Cayouette, Alice C. McHardy, Gordana Djordjevic, Nahla Aboushadi, Rotem Sorek, Susannah G. Tringe, Mircea Podar, Hector Garcia Martin, Victor Kunin, Daniel Dalevi, Julita Madejska, Edward Kirton, Darren Platt, Ernest Szeto, Asaf Salamov, Kerrie Barry, Natalia Mikhailova, Nikos C. Kyrpides, Eric G. Matson, Elizabeth A. Ottesen, Xinning Zhang, Myriam Hernández, Catalina Murillo, Luis G. Acosta, Isidore Rigoutsos, Giselle Tamayo, Brian D. Green, Cathy Chang, Edward M. Rubin, Eric J. Mathur, Dan E. Robertson, Philip Hugenholtz, Jared R. Leadbetter, Metagenomic and functional analysis of hindgut microbiota of a wood-feeding higher termite. Nature. ,vol. 450, pp. 560- 565 ,(2007) , 10.1038/NATURE06269
Margaret McFall-Ngai, Michael G. Hadfield, Thomas C. G. Bosch, Hannah V. Carey, Tomislav Domazet-Lošo, Angela E. Douglas, Nicole Dubilier, Gerard Eberl, Tadashi Fukami, Scott F. Gilbert, Ute Hentschel, Nicole King, Staffan Kjelleberg, Andrew H. Knoll, Natacha Kremer, Sarkis K. Mazmanian, Jessica L. Metcalf, Kenneth Nealson, Naomi E. Pierce, John F. Rawls, Ann Reid, Edward G. Ruby, Mary Rumpho, Jon G. Sanders, Diethard Tautz, Jennifer J. Wernegreen, Animals in a bacterial world, a new imperative for the life sciences Proceedings of the National Academy of Sciences of the United States of America. ,vol. 110, pp. 3229- 3236 ,(2013) , 10.1073/PNAS.1218525110
Stefan Götz, Juan Miguel García-Gómez, Javier Terol, Tim D Williams, Shivashankar H Nagaraj, María José Nueda, Montserrat Robles, Manuel Talón, Joaquín Dopazo, Ana Conesa, None, High-throughput functional annotation and data mining with the Blast2GO suite. Nucleic Acids Research. ,vol. 36, pp. 3420- 3435 ,(2008) , 10.1093/NAR/GKN176