Ankyrin-G coordinates assembly of the spectrin-based membrane skeleton, voltage-gated sodium channels, and L1 CAMs at Purkinje neuron initial segments

摘要: The axon initial segment is an excitable membrane highly enriched in voltage-gated sodium channels that integrates neuronal inputs and initiates action potentials. This study identifies Nav1.6 as the channel isoform at mature Purkinje neuron segments reports essential role for ankyrin-G coordinating physiological assembly of Nav1.6, βIV spectrin, L1 cell adhesion molecules (L1 CAMs) neurofascin NrCAM cerebellar neurons. Ankyrin-G spectrin appear by postnatal day 2, whereas CAMs are not fully assembled continuous high density along until 9. therefore do initiate protein segments. clustered adult mice lacking ankyrin-G. These results support conclusion coordinates a complex containing transmembrane molecules, channels, skeleton