Dome formation by oral epithelia in vitro.

摘要: Multicellular, cystic structures, termed domes, have been described previously in epithelia cultured from various tissues that a known transport or secretory function vivo and vitro. We report for the first time dome formation cells “covering” “rest” of oral tissues: porcine gingival alveolar mucosa epithelium epithelial rests Malassez. As demonstrated by light- electron microscopy, morphology domes varied with location their lumen number cell layers involved structure. Sequential observations using phase contrast microscopy lapse cinematography living cultures showed were dynamic structures expansion-collapse cycles between 30 min 17 h duration. Dome was stimulated dibutyryl cyclic AMP (dbcAMP, 10−3 to 10−6M) abolished ouabain (10−10M), an inhibitor sodium transport.