Silver, zinc oxide and titanium dioxide nanoparticle ecotoxicity to bioluminescent Pseudomonas putida in laboratory medium and artificial wastewater

摘要: abstract Bacteria based ecotoxicology assessment of manufactured nanoparticles is largely restricted to Escher-ichia coli bioreporters in laboratory media. Here, toxicity effects model OECD (Ag NM-300K, ZnO NM-110 and TiO 2 NM-104) were assessed using the switch-off luminescent Pseudomonasputida BS566::luxCDABE bioreporter Luria Bertani (LB) medium artificial wastewater (AW). IC 50 values ~4 mg L 1 ,100 >200 at h observed LB for Ag NM-110and NM-104, respectively. Similar results obtained AW NM-300K (IC ~5 )and NM-104 ) whereas was significantly higher ).Lower compared associated with differences agglomerationstatus dissolution rate. This work demonstrates importance nanoecotoxicological studies inenvironmentally relevant matrices.© 2014 Elsevier Ltd. All rights reserved. 1. IntroductionManufactured (NPs), defined as anthropogenicmaterials exhibiting all three external dimensions nanoscale(i.e. between 100 nm), are being increasingly used com-mercial products industrial processes due new or enhancedphysico-chemical properties their bulk counterparts(Ju-Nam Lead, 2008). Despite offering benefits, wide use ofNPs has raised questions regarding potential release andimpact humans environment (Peralta-Videa et al., 2011;Scown 2010). However, toxicological more specificallyecotoxicological NPs still limited such thepotential adverse remain unknown. Conse-quently, nanoparticle science leading interfacialmaterials (i.e. materials birthed by meeting two different parentmaterials atomic level), sciencecurrently lags behind developing suitable tools approachesfor identifying fate effect parent (Kahru Ivask,2013; Kim 2012; Klaine 2008).A key issue safety isthe appropriateness standard protocols (such ISO)to assess hazard. In this study we focus on ofthe most widely NPs, namely silver (Ag), zinc oxide (ZnO)and titanium dioxide (TiO