Studies on the Binding and Distribution of Radioactively Labelled 3′-Methylcholanthrene in Subcellular Fractions of Rat Liver

摘要: Abstract The subcellular distribution of either [14C] or [3H]3′-methylcholanthrene was studied in rat liver following a single intraperitoneal injection the labelled hydrocarbon 10 hours previously. Adsorbed non-covalently bound methylcholanthrene and its metabolic derivatives occurred all cell fractions with exception purified walls. The highest specific activities (d.p.m./mg. protein) were found washed mitochondria, microsomes ribosome-free microsomal membranes. Covalent binding to different occurs small extent is considered not be significant. degree microsomes, membranes their constituent core proteins. Cell sap which contains 3′-methylcholanthrene fractionated into pH 5 enzyme supernatant fractions. fraction possesses high activity further ammonium sulphate three 0-30% had activity.