Crystallization of wild-type and mutant ferricytochromes c at low ionic strength: seeding technique and X-ray diffraction analysis.

摘要: Ferricytochromes c were crystallized at low ionic strength by macroseeding techniques. Large crystals grown seed-induced self-nucleation which occurred anywhere in the drop, regardless of location seed crystal. This unusual crystal-seeding method worked reproducibly our hands, and X-ray quality have been prepared several ferricytochromes c: horse, rat (recombinant wild type), two site-directed mutants latter, tyrosine 67 to phenylalanine (Y67F) asparagine 52 isoleucine (N52I). Crystals any one these four proteins could be used as seeds for crystallization others. All are same crystal form, with space group P212121. There protein molecules per asymmetric unit. The stable beam diffract least 2.0 A, resolution. Full crystallographic data sets collected from single all proteins.