Mechanotransduction in bone: osteoblasts are more responsive to fluid forces than mechanical strain.

摘要: Mechanical force applied to bone produces two localized mechanical signals on the cell: deformation of extracellular matrix (substrate strain) and fluid flow. To study effects these stimuli osteoblasts, MC3T3-E1 cells were grown type I collagen-coated plastic plates subjected four-point bending. This technique uniform levels physiological strain forces cells. Each parameters can be varied independently. Osteopontin (OPN) mRNA expression was used assess anabolic response When low, neither magnitude nor rate correlated with OPN expression. However, higher-magnitude significantly increased message independently or rate. These data indicate that forces, not stretch, influence in osteoblasts suggest induced by flow within may play an important role formation loading.