Human umbilical cord mesenchymal stem cells derived from Wharton's jelly differentiate into insulin-producing cells in vitro.

摘要: BACKGROUND Islet transplantation is an effective way of reversing type I diabetes. However, islet hampered by issues such as immune rejection and shortage donor islets. Mesenchymal stem cells can differentiate into insulin-producing cells. the potential human umbilical cord mesenchymal (huMSCs) to become remains undetermined. METHODS We isolated induced cultured huMSCs under cell culture conditions. The response were monitored inverted phase contrast microscope. Immunocytochemical immunofluorescence staining methods used measure insulin glucagon protein levels. Reverse transcription-polymerase chain reaction (RT-PCR) was performed detect gene expression PDX-1. Dithizone-staining employed determine zinc contents in huMSCs. Insulin secretion also evaluated through radioimmunoassay. RESULTS HuMSCs nicotinamide β-mercaptoethanol or neurogenic differentiation 1 (NeuroD1) transfection gradually changed morphology from typically elongated fibroblast-shaped round They had a tendency form clusters. studies showed positive these induction. RT-PCR experiments found that expressed PDX-1 genes following induction dithizone stained cytoplasm brownish red color after significantly elevated compared with control group (t = 6.183, P < 0.05). CONCLUSIONS are able vitro. use β replacement therapy diabetes needs be studied further.