Enantioselective properties of extracellular lipase from Serratia marcescens ES-2 for kinetic resolution of (S)-flurbiprofen
作者： Hyun-Ae Bae , Kwang-Woo Lee , Yong-Hyun Lee
DOI: 10.1016/J.MOLCATB.2006.02.004
关键词: Serratia marcescens 、 Lipase 、 Hydrolysis 、 Kinetic resolution 、 Triacylglycerol lipase 、 Aqueous two-phase system 、 Enantiomeric excess 、 Chemistry 、 Yield (chemistry) 、 Chromatography
摘要: Abstract Serratia marcescens ES-2 excreting a catalytic lipase for the enantioselective hydrolysis of ( R , S )-flurbiprofen ethyl ester to was newly screened, and excreted purified by three-step procedure. The molecular mass measured be 64.9 kDa, relatively high value among known bacterial lipases, optimal temperature determined 37 °C. enantioselectivity significantly enhanced inorganic ion Ca 2+ surfactant Triton X-207, yet seriously inhibited EDTA SDS. kinetic resolution 50 mM optically pure carried out using 100 U lipase/mmol in an aqueous phase reaction system supplemented with 10 mM 1% X-207. A conversion, corresponding enantiomeric excess 98.5% conversion yield 45.1%, achieved after 24 h, along very E- 332. An from S. seems practically applicable production active )-flurbiprofen.
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