Electrophoretic and immunological evidence of unique proteins in leaves of citrus trees: application to citrus blight detection.

摘要: From the leaf tissue of healthy and blighted citrus trees 10–30 kDa soluble fractions were compared to find biochemical markers in disease state. Using a non-denaturing extrction technique coupled with ultrafiltration, resulting blight fraction was sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Two distinct adjacent bands for at an Mr approximately 12 500 separated. These visible Coomassie Brilliant Blue silver stain but negative glycoprotein stains. An antiserum prepared against proteins isolated by preparative reacted only distinctly different from when Western blotted. Only region (Mr 500–13 000) sources positive nondisease stress sources. Results indicate that identification specific may be way diagnose onset prior tree symptoms.