In situ forming hydrogel composed of hyaluronate and polygalacturonic acid for prevention of peridural fibrosis.

摘要: Hyaluronic acid-based hydrogels can reduce postoperative adhesion. However, the long-term application of hyaluronic acid is limited by tissue mediated enzymatic degradation. To overcome this limitation, we developed a polygalacturonic and hyaluronate composite hydrogel Schiff’s base crosslinking reaction. The had short gelation time (less than 15 s) degraded less 50 % in presence hyaluronidase for 7 days. Cell adhesion migration assays showed prevented fibroblasts from infiltration into hydrogels. Compared to commercial Medishield™ gels, was not totally vivo after 4 weeks. In rat laminectomy model, also better grade smaller mean area fibrous formation over saline control groups. Polygalacturonic system that be easy use due its situ cross-linkable property potentially promising prevention spine surgeries.