Rapid lactate oxidase‐based assay for lactate content in milk to ascertain its hygienic quality
作者： Brajesh Kumar , Rajan Sharma , Rahul Thakur , Amit Kumar Barui , Yudhishthir S Rajput
关键词: Lactate oxidase 、 Absorbance 、 Peroxidase 、 Hygienic quality 、 Chromatography 、 Magenta 、 Chemistry 、 Benzoic acid 、 Rapid assessment 、 Raw milk
摘要: A rapid method for qualitative and quantitative assaying of l-lactate in milk is described. The based on oxidation lactate by oxidase with the simultaneous formation H2O2 which subsequently oxidises 2, 4, 6-tribromo 3-hydroxy benzoic acid (TBHBA) presence peroxidase. Oxidised TBHBA then reacts 4-amino antipyrine to obtain magenta colour (λmax = 523 nm). In assay, change becomes visible raw samples spiked 300 mg lactate/L. absorbance increases linearly up 1000 mg/L. can be used assessment hygienic quality milk.
sci-hub.se 参考文章(21)
U. Müh, C.H. Williams, V. Massey, Lactate monooxygenase. II. Site-directed mutagenesis of the postulated active site base histidine 290. Journal of Biological Chemistry. ,vol. 269, pp. 7989- 7993 ,(1994) , 10.1016/S0021-9258(17)37149-1
N Shimojo, K Naka, C Nakajima, C Yoshikawa, K Okuda, K Okada, Test-strip method for measuring lactate in whole blood. Clinical Chemistry. ,vol. 35, pp. 1992- 1994 ,(1989) , 10.1093/CLINCHEM/35.9.1992
Wen-Jie Zhu, Da-Qian Feng, Meng Chen, Zhi-Dong Chen, Rong Zhu, Hai-Lin Fang, Wei Wang, Bienzyme colorimetric detection of glucose with self-calibration based on tree-shaped paper strip Sensors and Actuators B-chemical. ,vol. 190, pp. 414- 418 ,(2014) , 10.1016/J.SNB.2013.09.007
Neelam Upadhyay, Ankit Goyal, Anil Kumar, Darshan Lal Ghai, Richa Singh, None, Preservation of Milk and Milk Products for Analytical Purposes Food Reviews International. ,vol. 30, pp. 203- 224 ,(2014) , 10.1080/87559129.2014.913292
Ruitao Cha, David Wang, Zhibin He, Yonghao Ni, Development of cellulose paper testing strips for quick measurement of glucose using chromogen agent Carbohydrate Polymers. ,vol. 88, pp. 1414- 1419 ,(2012) , 10.1016/J.CARBPOL.2012.02.028
M.M. Rahman, Muhammad J.A. Shiddiky, Md. Aminur Rahman, Yoon-Bo Shim, A Lactate Biosensor Based on Lactate Dehydrogenase/Nictotinamide Adenine Dinucleotide (Oxidized Form) Immobilized on a Conducting Polymer/Multiwall Carbon Nanotube Composite Film Analytical Biochemistry. ,vol. 384, pp. 159- 165 ,(2009) , 10.1016/J.AB.2008.09.030
Rastislav Monošík, Miroslav Streďanský, Gabriel Greif, Ernest Šturdík, A rapid method for determination of l-lactic acid in real samples by amperometric biosensor utilizing nanocomposite Food Control. ,vol. 23, pp. 238- 244 ,(2012) , 10.1016/J.FOODCONT.2011.07.021
Rola Zaydan, Michel Dion, Mohammed Boujtita, Development of a new method, based on a bioreactor coupled with an L-lactate biosensor, toward the determination of a nonspecific inhibition of L-lactic acid production during milk fermentation. Journal of Agricultural and Food Chemistry. ,vol. 52, pp. 8- 14 ,(2004) , 10.1021/JF0343985
Ioannis Voulgaris, S. Alison Arnold, Robert Speight, Linda M. Harvey, Brian McNeil, Effects of dissolved oxygen availability and culture biomass at induction upon the intracellular expression of monoamine oxidase by recombinant E. coli in fed batch bioprocesses Process Biochemistry. ,vol. 46, pp. 721- 729 ,(2011) , 10.1016/J.PROCBIO.2010.11.019
Tianyi Jiang, Chao Gao, Cuiqing Ma, Ping Xu, Microbial lactate utilization: enzymes, pathogenesis, and regulation. Trends in Microbiology. ,vol. 22, pp. 589- 599 ,(2014) , 10.1016/J.TIM.2014.05.008