Genotypic analysis of Giardia duodenalis in domestic cats.

摘要: Background:Giardia duodenalis is an intestinal flagellated protozoan that affects many mammalian species often causing severe diarrheal disease. Several different genotypes have been identified (Assemblages A-G). Most isolates recovered from domestic cats assigned to either Assemblage A, the zoonotic form of parasite, or F, thus far only in cats. Genotypic variation within G. may influence clinical presentation and course Therefore, host-adapted not be responsible for disease (eg, F cats). Hypothesis:Multiple Giardia will present cats, including which correlated with signs. Animals:250 eastern Mississippi northwestern Alabama. Methods:Prevalence survey. Fecal samples evaluated cysts using a centrifugation concentration technique commercially available direct immunoflourescent antibody kit. were characterized by PCR amplification sequencing glutamate dehydrogenase gene. Results:Both A-I (6/17) (11/17) identified. Although was significantly associated age housing, no association detected between variety other factors presence gastrointestinal signs (acute vomiting, diarrhea, constipation). Conclusions Clinical Importance: The diarrhea cannot used as predictor animals study area. A veterinarians should consider any isolation potentially zoonotic.