Thoracic aortas from multiorgan donors are suitable for obtaining resident angiogenic mesenchymal stromal cells.

摘要: The clinical use of endothelial progenitor cells is hampered by difficulties in obtaining an adequate number functional progenitors. This study aimed to establish whether human thoracic aortas harvested from healthy multiorgan donors can be a valuable source angiogenic Immunohistochemical tissue studies showed that two distinct cell populations with putative stem capabilities, one composed CD34+ and the other c-kit+ cells, are present between media adventitia aortas. Ki-67+ high growth potential were located area corresponding site residence. We thus isolated (0.5 ∼ 2.0 × 104 aortic progenitors per 25 cm2) which, upon culturing, coexpressed molecules mesenchymal stromal (i.e., CD44+, CD90+, CD105+) transcript expression markers (e.g., OCT4, c-kit, BCRP-1, Interleukin-6) BMI-1. Cell expansion was for setting. A subset cultured acquired phenotype presence vascular factor increased KDR von Willebrand positivity), as documented flow cytometry, immunofluorescence, electron microscopy, reverse transcription-polymerase chain reaction assays. An vitro angiogenesis test kit revealed able form capillary-like structures within 6 hours seeding. demonstrates yield ability differentiate into cells. These used creation allogenic bank progenitors, providing new options restoring vascularization at ischemic sites. Disclosure conflicts interest found end this article.