Observation of through-hydrogen-bond 2hJHC' in a perdeuterated protein.
作者： Florence Cordier , Marco Rogowski , Stephan Grzesiek , Ad Bax
关键词: Hydrogen bond 、 Selective pulse 、 Analytical chemistry 、 Amide 、 Chemistry
摘要: It is demonstrated that J connectivity between amide protons and hydrogen-bond-accepting carbonyl carbons can be observed in perdeuterated human ubiquitin. A selective pulse scheme used to detect these small (2h)J(HC') interactions the presence of much larger through-covalent-bond (2)J(HC') (3)J(HC') couplings. The ratio through-H-bond correlation intensity a reference spectrum indicates values ca. 0.4-0.6 Hz, which are only slightly smaller than corresponding (3h)J(NC') values. However, for technical reasons, couplings more difficult measure (3h)J(NC'). Copyright 1999 Academic Press.
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