Purification and proteomics of pathogen-modified vacuoles and membranes.

摘要: Certain pathogenic bacteria adopt an intracellular lifestyle and proliferate in eukaryotic host cells. The niche protects the from cellular humoral components of mammalian immune system, at same time, allows to gain access otherwise restricted nutrient sources. Yet, protection nutrients comes with a price, i.e., need overcome cell-autonomous defense mechanisms, such as bactericidal endocytic pathway. While few rupture early phagosome escape into cytoplasm, most pathogens form distinct, degradation-resistant replication-permissive membranous compartment. Intracellular that unique pathogen vacuoles include Legionella, Mycobacterium, Chlamydia, Simkania, Salmonella species. In order understand formation these niches on global scale comprehensive quantitative manner, inventory compartment-associated factors is required. To this end, intact compartments be isolated, purified biochemically characterized. Here, we review recent progress isolation purification pathogen-modified membranes, well their proteomic characterization by mass spectrometry different validation approaches. These studies provide basis for further investigations specific mechanisms pathogen-driven compartment formation.