3'-terminal nucleoside unit containing phosphoramidite
作者： Akihiro Ohkubo , Kohji Seio , Mitsuo Sekine
DOI:
关键词: Nucleic acid 、 Derivative (chemistry) 、 A-DNA 、 Oligomer 、 Alkyl 、 Aryl 、 Phosphoramidite 、 Chemistry 、 Nucleoside 、 Stereochemistry
摘要: It is intended to provide a method of binding 3’-terminal nucleoside hydroxyl group on solid phase under the same conditions as in DNA chain extension reaction. A unit containing phosphoramidite which compound represented by following general formula (I): (N)-O-(R1)Si(R2)-(C6H4)-(CH2)n-O-P(OR3)N(R4)(R5) wherein (N) stands for an arbitrary or its derivative; R1, R2, R4 and R5 are each alkyl aryl group; R3 phosphate-protecting n integer from 1 5; support having unit, above compound, transferred thereinto; synthesizing nucleic acid oligomer with use this support.
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