Strain identification and quorum sensing inhibition characterization of marine-derived Rhizobium sp. NAO1

摘要: A novel strategy for combating pathogens is through the ongoing development and use of anti-quorum sensing (QS) treatments such as therapeutic bacteria or their anti-QS substances. Relatively little known about that inhabit open ocean potential anti-pathogenic attributes; thus, in an initiative to identify these types bacteria, planktonic microbes from North Atlantic Ocean were collected, isolated, cultured screened activity. Screening analysis identified one strain, Rhizobium sp. NAO1. Extracts NAO1 via ultra-performance liquid chromatography (UPLC) analysis. They shown contain N-acyl homoserine lactone (AHL)-based QS analogues (in particular, N-butyryl (C4-AHL) analogue) could disrupt biofilm formation by Pseudomonas aeruginosa PAO1. inhibition was confirmed using confocal scanning laser microscopy growth curves, it occur a dose-dependent manner without affecting bacterial growth. Secondary metabolites inhibited PAO1 pathogenicity downregulating AHL-mediated virulence factors elastase activity siderophore production. Furthermore, result structure damage, secondary metabolite products significantly increased sensitivity aminoglycoside antibiotics. Our results demonstrated strain has ability P. architecture, addition attenuating factor production pathogenicity. Therefore, newly ocean-derived serve inhibitor may be new microbial resource drug development.