Studies on human placental alkaline phosphatase
作者： Donald R. Harkness
DOI: 10.1016/0003-9861(68)90435-9
关键词: Extraction (chemistry) 、 Butanol 、 Chemistry 、 Placental alkaline phosphatase 、 Ammonium sulfate 、 Immunoelectrophoresis 、 Column chromatography 、 Zinc 、 Crystallization 、 Chromatography 、 Biophysics 、 Biochemistry 、 Molecular biology
摘要: Abstract Placental alkaline phosphatase has been purified by butanol extraction, methanol precipitation, and column chromatography on DEAE-cellulose crystallized from a solution 42% saturated with ammonium sulfate. The colorless crystals are needleshaped measure approximately 1 × 20 mμ. crystalline enzyme appeared homogeneous ultracentrifugation immunoelectrophoresis. spectrum of pure protein 280:260 ratio 1.93. average molecular weight several determinations was 125,000. Enrichment in zinc during purification demonstrated. amino acid analysis is reported.
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