Radioiodination of proteins in single polyacrylamide gel slices. Tryptic peptide analysis of all the major members of complex multicomponent systems using microgram quantities of total protein.
作者： J H Elder , R A Pickett , J Hampton , R A Lerner
DOI: 10.1016/S0021-9258(17)39987-8
关键词: Microgram 、 Total protein 、 Elution 、 Membrane protein 、 Multicomponent systems 、 Trypsin 、 Biochemistry 、 Chromatography 、 Dictyostelium discoideum 、 Polyacrylamide gel electrophoresis 、 Chemistry
摘要: A method is described for radioiodination to high specific activity of fixed and stained proteins within sodium dodecyl sulfate-polyacrylamide gels, without elution the from gel. Following radioiodination, can be removed gel by trypsin treatment peptides analyzed. This procedure offers a means structurally compare multicomponent systems when purification each component homogeneity unfeasible. Using this technique, we have compared tryptic all major protein components Moloney Rauscher leukemia viruses using only 50 100 microgram total virus. Additionally, analyzed membrane Dictyostelium discoideum at various stages in development. The validity technique its value as tool comparative studies identification precursor-product relationships discussed.
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