Mouse Models of Influenza Infection with Circulating Strains to Test Seasonal Vaccine Efficacy.

摘要: Influenza virus infection is a significant cause of morbidity and mortality worldwide. The surface antigens influenza change over time blunting both naturally acquired vaccine induced adaptive immune protection. Viral antigenic drift major contributing factor to the spread disease burden influenza. aim this study was develop better models using clinically relevant, strains test CB6F1 mice were infected with range viruses disease, inflammation, cell influx viral load characterised after infection. Infection circulating H1N1 representative B dose dependent response; however recent seasonal H3N2 did not any in mice, even at high titres. led recoverable virus, detectable by plaque assay RNA quantification infection, increased upper airway inflammation on day 7 comprised largely CD8 T cells. Having established models, immunised inactivated responses compared matched mismatched challenge strains. Whilst subtype strain recommended for use has remained constant seven seasons between 2010 2016, (2009 pandemic subtype) drifted genetically antigenically since 2009. To investigate effect observed protection, from A/California/7/2009 (H1N1) challenged recovered 2009, or 2015. Vaccination protected against either 2009 strains, but less effective 2015 strain. This reduction protection suggests that mouse vaccination can be used as an additional tool predict efficacy