Glycomics Approaches for the Bioassay and Structural Analysis of Heparin/Heparan Sulphates

摘要: The glycosaminoglycan heparan sulphate (HS) has a heterogeneous structure; evidence shows that specific structures may be responsible for functions in biological processes such as blood coagulation and regulation of growth factor signalling. This review summarises the different experimental tools methods developed to provide more rapid studying structure HS. Rapid sensitive facile purification HS, from tissue cell sources are reviewed. Data sets structural analysis often complex include multiple sample sets, therefore software have been HS data sets. These can readily applied chromatographic simplification (e.g., charge separation using strong anion exchange chromatography size gel filtration techniques. Finally, following sequencing human genome, research rapidly advanced with introduction high throughput technologies carry out simultaneous analyses many samples. Microarrays study macromolecular interactions (including glycan arrays) paved way bioassay which utilize arrays effects macromolecules on cells. Glycan described immobilisation techniques saccharides exploited develop platform probe responses signalling pathway activation. aims at reviewing available purification, systems “glycomics” approaches.