Immobilization of Proteins for Biosensor Development

作者: Elaine T. Vandenberg , R. Stephen Brown , Ulrich J. Krull

DOI: 10.1007/978-94-011-1334-2_2

关键词: Combinatorial chemistryBiosensorImmobilized proteinsSelectivityBiocompatibilitySolid surfaceDNA-binding proteinFunction (biology)MoleculeChemistry

摘要: There is considerable interest in the permanent attachment of bio-molecules to solid surfaces because many uses immobilized proteins: for purification molecules that bind antibodies, lectins or other binding proteins; solid-phase analytical assays; improved biocompatibility materials; and provide selectivity biosensors. In such applications, amount protein retention physiological activity (such as a second molecule) are interest. This work will report proteins function method immobilization, using instrumental techniques. The results all whose involves proteins, but study was inspired by its application field biosensors, evidenced choice (quartz silicon), types employed (those which molecule).

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