Purification and identification of recombinant hirudin and its degradation products expressed in Pichia pastoris.
作者： Wei‐Bin Zhou , Yuan‐Xing Zhang
DOI: 10.1081/PB-200026807
关键词: Recombinant Hirudin 、 Hirudin 、 Reversed-phase chromatography 、 Chemistry 、 Pichia pastoris 、 Proteolytic degradation 、 Degradation (geology) 、 Peptide sequence 、 Biochemistry 、 Chromatography 、 Yield (chemistry)
摘要: Abstract The purification and identification of recombinant hirudin (r‐hirudin) (rHV2‐Lys47) its several C‐terminal proteolytic degradation derivatives, produced by Pichia pastoris, were described. high‐purity rHV2‐Lys47 above 99% three products obtained a straightforward two‐step chromatography procedure, combination cation exchange reverse phase chromatography, with recovery yield 74% for hirudin. purified rHV2 had the predicted N‐terminal amino acid sequence derivatives hirudin, short one to residues at C‐terminal.
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