Uniformly spaced banding pattern in DNA sequencing gels by use of field-strength gradient
作者： Anders Olsson , Tomas Moks , Mathias Uhlén , Andras b. Gaal
DOI: 10.1016/0165-022X(84)90053-8
关键词: Sequencing data 、 Biology 、 Casting 、 Analytical chemistry 、 Polyacrylamide gel electrophoresis 、 Gel electrophoresis 、 DNA sequencing 、 Oligonucleotide 、 Field strength 、 Biophysics 、 Biochemistry
摘要: Abstract A method is described for casting and operating ultrathin wedge-shaped field-strength gradient gels DNA sequencing. One of the gradients results in a uniformly-spaced oligonucleotide banding pattern from 60 to 300 nucleotides 53 cm long 4% polyacrylamide gel. From these gels. more sequencing data can be obtained with greater confidence than uniform thickness. The gel-casting fast simple. At 2.5 kV two run simulatenously 2 h same power supply.
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