DEEP DIVISION IN THE CHLOROPHYCEAE (CHLOROPHYTA) REVEALED BY CHLOROPLAST PHYLOGENOMIC ANALYSES(1).

摘要: The Chlorophyceae (sensu Mattox and Stewart) is a morphologically diverse class of the Chlorophyta displaying biflagellate quadriflagellate motile cells with varying configurations flagellar apparatus. Phylogenetic analyses 18S rDNA data combined 26S from broad range chlorophycean taxa uncovered five major monophyletic groups (Chlamydomonadales, Sphaeropleales, Oedogoniales, Chaetophorales, Chaetopeltidales) but could not resolve their branching order. To gain insight into interrelationships these groups, we analyzed multiple genes encoded by chloroplast genomes Chlamydomonas reinhardtii P. A. Dang. moewusii Gerloff (Chlamydomonadales), Scenedesmus obliquus (Turpin) Kutz. (Sphaeropleales), Oedogonium cardiacum Wittr. (Oedogoniales), Stigeoclonium helveticum Vischer (Chaetophorales), Floydiella terrestris (Groover et Hofstetter) Friedl O’Kelly (Chaetopeltidales). C. moewusii, Oedogonium, DNAs were partly sequenced using random strategy. Trees reconstructed nucleotide amino acid sets derived 44 protein-coding 11 chlorophytes nine streptophytes as well 57 six taxa. All best trees identified two robustly supported lineages within Chlorophyceae: clade uniting Chlamydomonadales Chaetopeltidales (OCC clade). This dichotomy independently molecular signatures in genes, such insertions/deletions distribution trans-spliced group II introns. Within OCC clade, sister relationship observed for Chaetophorales also strengthened independent data. Character state reconstruction basal body orientation allowed us to refine hypotheses regarding evolution