Complete blood count, clinical chemistry, and serology profile by using a single tube of whole blood from mice.
作者： Craig Franklin , Charles E. Wiedmeyer , Dawn Ruben
DOI:
关键词: Anticoagulant 、 Pathology 、 Medicine 、 Cold storage 、 Whole blood 、 Clinical pathology 、 Nuclear medicine 、 Blood sampling 、 Blood chemistry 、 Complete blood count 、 Heparin
摘要: Clinical pathology is a valuable means for assessing specific organ and screening tool general animal health. Routine clinical evaluation in mice usually includes whole blood complete count (CBC) biochemistry analysis. Acquisition analysis of these samples can be problematic due to the small volumes that obtained from mouse. Typically, requires tube containing an anticoagulant, whereas profile needs serum clot tube. Because volume obtained, splitting single mouse into 2 different tubes may result inadequate perform desired tests or introduce inaccuracies. We explored feasibility using lithium heparin generation CBC, profile, serology profile. also evaluated consistency CBC data, including quality peripheral smear taken EDTA after various storage times. found biochemistry, profiles could more readily when were placed than separate tubes. In addition, smears results comparable (with few exceptions) those prolonged storage.
elsevier.com
researchgate.net 参考文章(10)
Bai-Hsiun Chen, Cheng-Hwa Chiang, Jen-Feng Fong, Effect of different anticoagulant, underfilling of blood sample and storage stability on selected hemogram. Kaohsiung Journal of Medical Sciences. ,vol. 15, pp. 87- 93 ,(1999) , 10.6452/KJMS.199902.0087
Diane E. McClure, Clinical pathology and sample collection in the laboratory rodent. Veterinary Clinics of North America: Exotic Animal Practice. ,vol. 2, pp. 565- 590 ,(1999) , 10.1016/S1094-9194(17)30111-1
Robert L. Hall, Clinical Pathology for Preclinical Safety Assessment: Current Global Guidelines Toxicologic Pathology. ,vol. 20, pp. 472- 476 ,(1992) , 10.1177/0192623392020003202
Synnøve Vatn, Tore Framstad, Wenche O. Torsteinbø, Hematologic Evaluation of Normal and Anemic Lambs with the Technicon H*1 Using EDTA or Heparin as Anticoagulants Veterinary Clinical Pathology. ,vol. 29, pp. 62- 68 ,(2000) , 10.1111/J.1939-165X.2000.TB00401.X
Dario A.A. Vignali, Multiplexed particle-based flow cytometric assays. Journal of Immunological Methods. ,vol. 243, pp. 243- 255 ,(2000) , 10.1016/S0022-1759(00)00238-6
C. Médaille, A. Briend-Marchal, J. P. Braun, Stability of selected hematology variables in canine blood kept at room temperature in EDTA for 24 and 48 hours. Veterinary Clinical Pathology. ,vol. 35, pp. 18- 23 ,(2006) , 10.1111/J.1939-165X.2006.TB00083.X
Kurt Weingand, John Bloom, Michael Carakostas, Robert Hall, Maria Helfrich, Kenneth Latimer, Barry Levine, Douglas Neptun, Alan Rebar, Katherine Stitzel, Catherine Troup, Clinical Pathology Testing Recommendations for Nonclinical Toxicity and Safety Studies Toxicologic Pathology. ,vol. 20, pp. 539- 543 ,(1992) , 10.1177/0192623392020003217
KURT WEINGAND, GEOFF BROWN, ROBERT HALL, DAI DAVIES, KENT GOSSETT, DOUG NEPTUN, TREVOR WANER, TOSHIAKI MATSUZAWA, PAUL SALEMINK, WILHELM FROELKE, JEAN-PIERRE PROVOST, GIANNI DAL NEGRO, JOHN BATCHELOR, MAMORU NOMURA, HORST GROETSCH, ALPHONS BOINK, JON KIMBALL, DAVID WOODMAN, MALCOLM YORK, EVA FABIANSON-JOHNSON, MICHEL LUPART, ELSA MELLONI, Harmonization of Animal Clinical Pathology Testing in Toxicity and Safety Studies Toxicological Sciences. ,vol. 29, pp. 198- 201 ,(1996) , 10.1093/TOXSCI/29.2.198
Jun Kanno, Clinical Pathology Testing Regulatory Concerns Toxicologic Pathology. ,vol. 20, pp. 534- 537 ,(1992) , 10.1177/0192623392020003215
Hiltje Irausquin, The value of clinical chemistry data in animal screening studies for safety evaluation. Toxicologic Pathology. ,vol. 20, pp. 515- 518 ,(1992) , 10.1177/0192623392020003210