Broadband single cell impedance spectroscopy using maximum length sequences: theoretical analysis and practical considerations

摘要: Measurements of the dielectric (or impedance) properties cells can be used as a general characterization and diagnostic tool. In this paper, we describe novel impedance spectroscopy technique for analysis single biological in suspension. The uses maximum length sequences (MLS) periodic excitation signal microfluidic cytometer. method allows multi-frequency cell measurements to made short time period (ms). Spectral information is obtained frequency domain by applying fast M-sequence transform (FMT) Fourier (FFT) response. Theoretically, determined from transfer function system when MLS current excitation. order sampling rate A/D conversion are two factors that determine bandwidth spectral accuracy technique. Experimentally, applicability demonstrated characterizing spectrum red blood (RBCs) measured within 1 ms at 512 discrete frequencies, evenly distributed range 976.56 Hz 500 kHz. shows good agreement with simulations.