Metabolic Engineering of Synechocystis sp. PCC 6803 for Production of the Plant Diterpenoid Manoyl Oxide
作者： Elias Englund , Johan Andersen-Ranberg , Rui Miao , Björn Hamberger , Pia Lindberg
DOI: 10.1021/ACSSYNBIO.5B00070
关键词: Enzyme 、 Metabolic engineering 、 Synechocystis 、 Coleus 、 Plectranthus 、 Forskolin 、 Biology 、 Terpenoid 、 Biochemistry 、 Chemical synthesis
摘要: Forskolin is a high value diterpenoid with a broad range of pharmaceutical applications, naturally found in root bark of the plant Coleus forskohlii. Because of its complex molecular structure, chemical synthesis of forskolin is not commercially attractive. Hence, the labor and resource intensive extraction and purification from C. forskohlii plants remains the current source of the compound. We have engineered the unicellular cyanobacterium Synechocystis sp. PCC 6803 to produce the forskolin precursor 13R-manoyl oxide (13R …
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