Enhanced production of diosgenin through elicitation in micro-tubers of Chlorophytum borivilianum Sant et Fernand

摘要: Abstract Diosgenin, one of the steroidal sapogenin, is a chief bioactive compound, commercially known for its pharmaceutical application to treat sexual dysfunction. The biosynthesis plant secondary metabolites in vitro cultures usually manipulated by different elicitors that lead higher production than non-elicited cultures. In present investigation, tubers Chlorophytum borivilianum were produced on semisolid and stationary liquid Murashige Skoog (MS) medium supplemented with various concentrations sucrose. To elicit diosgenin contents, these micro-tubers exposed jasmonic acid (JA) salicylic (SA) 1-month. An optimum number micro-tuber was obtained MS containing 60 g/L However, consisting same sucrose level found more suitable increased medium. Exposure lower doses JA SA individually induced improved C. borivilianum. A 2.1-fold after 1-month exposure 25 μM JA; while elicitation, 1.5-fold comparison mother plant. results suggest have considerable ability stimulate valuable