A Protocol for Isolation, Purification, Characterization, and Functional Dissection of Exosomes.
作者： David W Greening , David W Greening , Alin Rai , Richard J Simpson , Bethany Claridge
DOI: 10.1007/978-1-0716-1186-9_9
关键词: Proteomics 、 Drug delivery 、 Chemistry 、 RNA 、 Reprogramming 、 Cell 、 Cell biology 、 Microvesicles 、 Endocytic cycle 、 Secretory protein
摘要: Extracellular vesicles (EVs) are membrane-enclosed vesicles released by cells. They carry proteins, nucleic acids, and metabolites which can be transferred to a recipient cell, locally or at a distance, to elicit a functional response. Since their discovery over 30 years ago, the functional repertoire of EVs in both physiological (eg, organ morphogenesis, embryo implantation) and pathological (eg, cancer, neurodegeneration) conditions has cemented their crucial role in intercellular communication. Moreover, because the cargo encapsulated …
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