Chapter 31 Transport of DNA through bacterial membranes

摘要: Publisher Summary DNA translocation during injection of phage into prokaryotic cells proceeds with an extraordinary high rate 10,000 base-pairs per second (estimated for T4). rates in conjugation and natural transformation are estimated to be much slower: the order a 1000 100 bases respectively. This chapter discusses specificity uptake systems their substrates (example: sequence, minimal length, single- vs. double stranded forms, DNA/RNA hybrids) orientation which these molecules enter cell. may great importance subsequent experiments on purification system. To know diameter system, two approaches presented: (1) attachment bulky (protein) substrate increasing (2) single-channel recordings ion fluxes through channel. The latter approach is used identification reconstituted mitochondrial peptide channel, whereas former applied Acinetobacter DNA-translocase.