Generation of Marker-Free pbd-2 Knock-in Pigs Using the CRISPR/Cas9 and Cre/loxP Systems.
作者： Jing Huang , Antian Wang , Chao Huang , Yufan Sun , Bingxiao Song
关键词: CRISPR 、 Selectable marker 、 Cre recombinase 、 Transgene 、 Cre-Lox recombination 、 Gene knockin 、 Cas9 、 Gene 、 Biology 、 Molecular biology
摘要: … knock-in events were identified by PCR using three primer pairs (Table S1) and the positive cell … against different pathogens including bacteria, fungi and viruses. Our previous in vivo …
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