An Application of the Frozen Sectioning Technic for Cutting Serial Sections Thru the Brain

摘要: The availability of CO2 ice makes it practical to cut large blocks cerebral tissue by the freezing method. If is first treated with 20–30% ethyl alcohol for sufficient time secure uniform penetration (about 24 hours), formation hard crystals can be controlled and serial sections 25–100 μ thick negligible loss. added fixative used perfusion, or at any later in firing process, after fixation completed. are cemented slide groups slides manipulated thru staining processes glass trays. Ordinary cell fiber stains give satisfactory results. method particularly useful certain neurophysiological purposes such as defining location electrode tracks lesions types retrogrades. Prussian blue test electrolytically deposited iron conveniently applied conjunction other stains, determine point which a g...