Edema and cell infiltration in the phorbol ester-treated mouse ear are temporally separate and can be differentially modulated by pharmacologic agents.

摘要: The temporal patterns of edema and accumulation the PMN marker enzyme, myeloperoxidase (MPO), were examined following application tetradecanoylphorbol acetate (TPA) to mouse ears. After 2.5 micrograms TPA, peaked at 6 hr, while MPO activity 24 hr. Pharmacological agents with defined mechanisms action, delivered orally or topically, assessed for effects on these responses. For oral administration, compounds 1 hr before after TPA topical administration 15 min TPA. Topical corticosteroids inhibited both accumulation. Cyclooxygenase lipoxygenase inhibitors very effective against but either inactive moderately active vs edema. Anti-histamine/anti-serotonin had little effect edema, could inhibit exacerbate depending dose route administration. Topically applied histamine itself did not TPA-induced markedly suppressed Acetone, vehicle, when topically between 0.5 2 by 60-80%, Acetone no parameter. These results indicate that in ear inflammation model response may be a useful additional indicator drug activity.