High-resolution polyacrylamide gel electrophoresis of fluorophore-labeled reducing saccharides.

摘要: Publisher Summary The polyacrylamide gel electrophoresis (PAGE), which has become the standard and ubiquitous method for analytical separation of large molecules such as proteins DNA sequencing, is used in important ways carbohydrates. fluorophore-derivatized saccharides are analyzed by PAGE. most elements outlined chapter. key to high resolution, obtained with 8-aminonaphthalene-1,3,6-trisulfonic acid (ANTS) charged that have been derivatized 2-aminoacridone, lies electrophoretic buffer systems used. A wide variety monosaccharides, oligosaccharides, small polysaccharides separated In particular, ANTS derivatives resolution enables some pairs isomers, anomers, epimers. PAGE system designed specifically ANTS-derivatized oligosaccharides available commercially. includes precast gels, prepacked buffers, an apparatus, a power supply unit. recommended its convenience resolving because cassettes be gel-imaging system.