Molecular characterization of an anti-epilepsy peptide from the scorpion Buthus martensi Karsch
作者： Chun-Guang Wang , Xiao-Lin He , Feng Shao , Wei Liu , Min-Hua Ling
DOI: 10.1046/J.1432-1327.2001.02132.X
关键词: Neurotoxin 、 Peptide 、 Molecular biology 、 Peptide sequence 、 Biology 、 Amino acid 、 Edman degradation 、 Scorpion 、 Sequence analysis 、 Signal peptide 、 Biochemistry
摘要: For a long time Asian scorpion Buthus martensi Karsch (BmK) has been used in Chinese traditional medicine to cure many diseases of nervous system. Here we report the purification and characterization pharmacologically active neurotoxin from BmK. This toxin had little toxicity mice insects but was found have an anti-epilepsy effect rats, is thus named as BmK peptide (BmK AEP). Its amino-acid sequence determined by lysylendopeptidase digestion, Edman degradation mass spectrographic analysis. Based on sequence, gene coding for this also cloned sequenced 3' 5' RACE methods. It encodes precursor 85 residues including signal 21 residues, mature 61 three additional Gly-Lys-Lys at C-terminus. The Gly sometimes followed one or two basic prerequisite amidation its C-terminal verified molecular-mass determination AEP. shares homology with other depressant insect toxins. remarkable difference between them mainly focused 6, 7 39; these might relate unique action
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