Osteoblast/fibroblast coculture derived bioactive ECM with unique matrisome profile facilitates bone regeneration.
作者： Mei Li , Anqi Zhang , Jiajing Li , Jing Zhou , Yanan Zheng
DOI: 10.1016/J.BIOACTMAT.2020.06.017
关键词: Tissue engineering 、 In vitro 、 Osteoblast 、 Chemistry 、 Bone regeneration 、 In vivo 、 Fibroblast 、 Extracellular matrix 、 Regenerative medicine 、 Cell biology
摘要: Extracellular matrix (ECM) with mimetic tissue niches was attractive to facilitate regeneration in situ via recruitment of endogenous cells and stimulation self-healing process. However, how engineer the complicate specific ECM unique matrisome vitro a challenge ECM-based biomaterials engineering regenerative medicine. Here, we introduced coculture system bone niche guided by cell-cell communication. In cocultures, fibroblasts promoted osteogenic differentiation osteoblasts extracellular vesicles. The generated (MN-ECM) displayed appearance morphology biological components. advantages MN-ECM were demonstrated promotion multiple cellular behaviors (proliferation, adhesion mineralization) vivo. Moreover, proteomic analysis used clarify molecular mechanism MN-ECM, which revealed signature. present study provides novel strategy generate communication system, forwards development tissue-bioactive along deepening understanding regulated for engineering.
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