Recombinant Retroviruses Pseudotyped With the Vesicular Stomatitis Virus G Glycoprotein Mediate Both Stable Gene Transfer and Pseudotransduction in Human Peripheral Blood Lymphocytes
作者： H.F. Gallardo , C. Tan , D. Ory , M. Sadelain
关键词: Rhabdoviridae 、 Virus 、 Transfection 、 CD8 、 Biology 、 Virology 、 Genetic transfer 、 Vesicular stomatitis virus 、 Vesicular stomatitis Indiana virus 、 Transduction (genetics)
摘要: It is essential for the study of T-cell function and improvement adoptive cell therapies to efficiently generate large populations human primary T cells that reliably express foreign genes. This goal achieved by using recombinant retroviruses pseudotyped with either gibbon ape leukemia virus (GaLV) envelope or vesicular stomatitis G (VSV-G) glycoprotein. We show here both retroviral particles mediate stable gene transfer in CD4+ CD8+ peripheral blood lymphocytes cultured under optimized conditions. However, VSV-G-pseudotyped virions may cause transduction artifacts must be carefully excluded. The VSV-G require 10- 100-fold higher concentrations infectious achieve levels comparable GaLV-virions. Nonetheless, physical stability VSV-G-coated enables concentration viral stocks 10(9) per milliliter more.
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