Rapid quantification of HTLV-I provirus load: detection of monoclonal proliferation of HTLV-I-infected cells among blood donors.

摘要: In this report, we quantified HTLV-I provirus load using the AmpliSensor system, which utilizes fluorescence to measure PCR products. With method, loads could be measured within 6 hr, and results obtained correlated well with those by other methods. Samples from 256 blood donors, who were positive for antibodies against HTLV-I, analyzed, showing that ranged less than 0.1% 56% among carriers. We analyzed association between biomarkers age sex found it was not influenced either. Provirus better soluble interleukin-2 receptor (sIL-2R) levels antibody titer virus. Among 18 donors high (more 10%), Southern blotting detected monoclonal integration of in infected cells 2 cases, both them sIL-2R 900 U/ml). Sequential analyses showed stable same carriers, suggesting some factors or determined individuals. Thus, rapid method is a useful tool early detection adult T-cell leukemia HTLV-I-associated diseases.