Effects of nitrogenous nutrition on growth and nitrogen assimilation enzymes of dinoflagellate Akashiwo sanguinea
作者： Yun Liu , Tiantian Chen , Shuqun Song , Caiwen Li
DOI: 10.1016/J.HAL.2015.10.005
关键词: Botany 、 Akashiwo sanguinea 、 Urea 、 Biochemistry 、 Dinoflagellate 、 Biology 、 Urease 、 Nitrate reductase 、 Nitrogen assimilation 、 Assimilation (biology) 、 Algal bloom
摘要: The dinoflagellate Akashiwo sanguinea is a cosmopolitan species which frequently causes dense blooms in estuaries and coasts around the world, while mechanism regulating formation of algal bloom remains unclear. To further explore physiological response A. sartguinea during blooms, we studied effects various nitrogenous nutrients on growth nitrogen assimilation enzymes laboratory trials. Our results indicated that was able to grow well multiple N substrates within appropriate ranges concentrations. grew faster NH4+, it showed greatest affinity for urea. intracellular were regulated adapt environmental nutrient conditions stages: nitrate reductase. (NR) induced when grown NO inhibited by NH4+ urea, glutamine synthetase (GS) expressed constitutively all sources, urease up-regulated NO3- urea but down-regulated NH4+. Meanwhile, activities decreased with sanguinea, GS activity significant positive correlation rates. extremely high assessed kinetics assimilation, indicating its competitive advantage over other phytoplankton species. facilitates utilization reduced N, implies capability forming environments where common source. (C) 2015 Elsevier B.V. All rights reserved.
qdio.ac.cn参考文章(65)
Tiantian Chen, Yun Liu, Shuqun Song, Caiwen Li, Ying Zhong Tang, Zhiming Yu, The effects of major environmental factors and nutrient limitation on growth and encystment of planktonic dinoflagellate Akashiwo sanguinea Harmful Algae. ,vol. 46, pp. 62- 70 ,(2015) , 10.1016/J.HAL.2015.05.006
Jacques Monod, Recherches sur la croissance des cultures bactériennes Hermann. ,(1942)
TAV. Rees, T. R. Larson, JWG. Heldens, FGJ. Huning, In Situ Glutamine Synthetase Activity in a Marine Unicellular Alga (Development of a Sensitive Colorimetric Assay and the Effects of Nitrogen Status on Enzyme Activity) Plant Physiology. ,vol. 109, pp. 1405- 1410 ,(1995) , 10.1104/PP.109.4.1405
C. Fan, P. M. Glibert, J. Alexander, M. W. Lomas, Characterization of urease activity in three marine phytoplankton species, Aureococcus anophagefferens, Prorocentrum minimum, and Thalassiosira weissflogii Marine Biology. ,vol. 142, pp. 949- 958 ,(2003) , 10.1007/S00227-003-1017-8
P. M. Glibert, C. Legrand, The Diverse Nutrient Strategies of Harmful Algae: Focus on Osmotrophy Ecological Studies. pp. 163- 175 ,(2006) , 10.1007/978-3-540-32210-8_13
Zhao ZengXia, Jiao NianZhi, Zhao WeiHong, Forms of nitrogen in the Yantai Sishili Bay cultivated water. Oceanologia et Limnologia Sinica. ,vol. 31, pp. 53- 59 ,(2000)
Susan Badylak, Edward J. Phlips, A. Loren Mathews, Akashiwo sanguinea (Dinophyceae) blooms in a sub-tropical estuary: An alga for all seasons Plankton and Benthos Research. ,vol. 9, pp. 147- 155 ,(2014) , 10.3800/PBR.9.147
Zhangxi Hu, Shunshan Duan, Ning Xu, Margaret R. Mulholland, Growth and Nitrogen Uptake Kinetics in Cultured Prorocentrum donghaiense PLOS ONE. ,vol. 9, ,(2014) , 10.1371/JOURNAL.PONE.0094030
CM Solomon, PM Glibert, Urease activity in five phytoplankton species Aquatic Microbial Ecology. ,vol. 52, pp. 149- 157 ,(2008) , 10.3354/AME01213
Claire Maurin, Yves Le Gal, Glutamine synthetase in the marine coccolithophorid Emiliania huxleyi (Prymnesiophyceae): regulation of activity in relation to light and nitrogen availability Plant Science. ,vol. 122, pp. 61- 69 ,(1997) , 10.1016/S0168-9452(96)04539-6