Changes in the K+ current-density of MCF-7 cells during progression through the cell cycle: possible involvement of a h-ether.a-gogo K+ channel.
作者： Ouadid-Ahidouch H , Prevarskaya N , Delcourt P , Toillon Ra , Roudbaraki M
DOI:
关键词: Cell cycle 、 Cell growth 、 Channel blocker 、 Depolarization 、 Chemistry 、 Membrane potential 、 Tetraethylammonium 、 Molecular biology 、 Astemizole 、 Extracellular
摘要: MCF-7 cells express voltage-activated K+ channels. In the present study, we used patch-clamp and RT-PCR techniques to investigate involvement of these channels during cell cycle progression. The outward rectifier current (IK) recorded depolarization was almost completely suppressed by classical channel blocker tetraethylammonium (TEA) in cells. TEA also inhibited proliferation, as measured with 3H-thymidine incorporation. Moreover, profound changes were observed both resting membrane potential (RMP) IK release from G0/G1 phase cycle. arrested depolarized (-26.3 +/- 10 mV, n = 30) IK-density small (9.4 5.6 pA/pF, 60) compared progressing G1 (RMP -60 7.9 mV; 35 30.2 8.5 pA/pF; 76). highly sensitive Mg2+, astemizole (10 mM). Extracellular perfusion 5 mM Mg2+ dramatically slowed activation 2 microM (20 3%) proliferation (23%). h-EAG mRNA expression modulated Thus, data suggested that play a role controlling and/or
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