Importance of platelet-free preparations for evaluating lymphocyte nucleotide levels in inherited or acquired immunodeficiency syndromes.

摘要: Low ATP/ADP ratios have been reported consistently for nucleotide levels of mononuclear cells separated from peripheral blood by conventional techniques. We established that these low values (mean 2.3:1) were not due to cell damage or poor viability, but resulted heavy platelet contamination, which is unavoidable when heparinized used. The results reflect the 1.6:1) characteristic platelets. Platelet-free extracts defibrinated had very high 17.4:1). initial finding detectable amounts deoxy-ATP and deoxy-GTP in children with two distinct inherited immunodeficiency disorders [adenosine deaminase (ADA) purine nucleoside phosphorylase (PNP) deficiency respectively] many contamination nucleated erythrocytes as well platelets non-defibrinated preparations. Defibrination before extraction a patient T-cell leukaemic/lymphoma treated ADA inhibitor deoxycoformycin enabled demonstration grossly raised relative deoxy-ADP (ratio 16.1:1), associated severe ATP depletion. This reciprocal relationship between dATP was found us previously deficiency. These findings underline importance uncontaminated platelets, erythrocytes, evaluation lymphocyte acquired syndromes.