Microscale NMR Screening of New Detergents for Membrane Protein Structural Biology
作者： Qinghai Zhang , Reto Horst , Michael Geralt , Xingquan Ma , Wen-Xu Hong
DOI: 10.1021/JA077863D
关键词: Integral membrane protein 、 Bacterial outer membrane 、 Electrophoresis 、 Peripheral membrane protein 、 Chemistry 、 Biochemistry 、 Phosphocholine 、 Structural biology 、 Membrane protein 、 Protein folding
摘要: The rate limiting step in biophysical characterization of membrane proteins is often the availability suitable amounts protein material. It was therefore interest to demonstrate that microcoil nuclear magnetic resonance (NMR) technology can be used screen microscale quantities for proper folding samples destined structural studies. Micoscale NMR then a series newly designed zwitterionic phosphocholine detergents their ability reconstitute proteins, using previously well characterized β-barrel E. coli outer OmpX as test case. Fold screening thus achieved with microgram uniformly 2H,15N-labeld and affordable detergents, prescreening SDS-gel electrophoresis ensured efficient selection targets A systematic approach optimize motif refolding led identification two new 138-Fos 1...
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