전복 용매 추출물의 세포독성과 항산화 활성
作者： Sun Young Lim
DOI: 10.5352/JLS.2014.24.7.737
关键词: Cytotoxicity 、 Methanol 、 Butanol 、 Haliotis discus 、 Viability assay 、 Chemistry 、 Abalone 、 Antioxidant 、 Biochemistry 、 Chromatography 、 Acetone
摘要: The objective of this study was to investigate the fatty acid composition raw and dried abalone (Haliotis discus hannai) determine effect extracts on cytotoxic activity antioxidant properties. Dried extracted with acetone/methylene chloride (A+M) methanol (MeOH), were fractionated using n-hexane, 85% aq. butanol (BuOH), water. Cytotoxic against HT-29 cancer cell lines determined 3-(4,5-dimethylthiazol)-2,5-diphenyltetrazolium bromide (MTT) assay. Antioxidant measured a fluorescence sensitive dye, 2’-7’ dichlorofluorescein-diacetate (DCFH-DA). higher (22:6n-3) than that abalone, it had lower percentage 20:4n-6 abalone. Analysis viability showed crude extract treatments fractions cytotoxic, suppressing growth (p<0.05). A+M cells compared MeOH extract. Among fractions, fraction strongest cytotoxicity cells. highest in terms scavenging reactive oxygen species (ROS) likewise obtained use MeOH. Our results suggest has potent inhibitory proliferation human
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